#Probiotic Properties of #Escherichia coli Nissle in Human Intestinal Organoids

Probiotic, or beneficial, bacteria, such as E. coli Nissle, hold promise for the treatment of human disease. More study is needed to fully realize the potential of probiotics. Safety and efficacy studies are critically important; however, mice are poor models for many human intestinal diseases. We used stem cell-derived human intestinal organoid tissues to evaluate the safety of Nissle and its ability to protect from … Continue reading #Probiotic Properties of #Escherichia coli Nissle in Human Intestinal Organoids

Food & mood: a review of supplementary prebiotic and probiotic interventions in the treatment of anxiety and depression in adult

A bidirectional relationship exists between the brain and the gastrointestinal tract. Foods containing bacteria that positively influence the gastrointestinal microbiome are termed, probiotics; compounds that promote the flourishing of these bacteria are termed, prebiotics. Whether microbiome influencing therapies could treat psychiatric conditions, including depression and anxiety, is an area of interest. Presently, no established consensus for such treatment exists.. .. 7 studies were identified. All … Continue reading Food & mood: a review of supplementary prebiotic and probiotic interventions in the treatment of anxiety and depression in adult

Reservoirs of antimicrobial resistance genes in retail raw milk

Background It has been estimated that at least 3% of the USA population consumes unpasteurized (raw) milk from animal sources, and the demand to legalize raw milk sales continues to increase. However, consumption of raw milk can cause foodborne illness and be a source of bacteria containing transferrable antimicrobial resistance genes (ARGs). To obtain a comprehensive understanding of the microbiome and antibiotic resistome in both … Continue reading Reservoirs of antimicrobial resistance genes in retail raw milk

Environmental Factors and Host Microbiomes Shape Host–Pathogen Dynamics

In many cases, interactions among host, pathogen, and environment are insufficient to describe disease dynamics in the wild. The host microbiome plays an important role in host immunity, at the interface between the host and the pathogen, and affects disease outcome. The environment, as well as environmental microbiomes, influence the host microbiome, and also influence simultaneously the host and the pathogen. The three-edged disease triangle … Continue reading Environmental Factors and Host Microbiomes Shape Host–Pathogen Dynamics

Permissive microbiome characterizes human subjects with a neurovascular disease cavernous angioma

Cavernous angiomas (CA) are common vascular anomalies causing brain hemorrhage. Based on mouse studies, roles of gram-negative bacteria and altered intestinal homeostasis have been implicated in CA pathogenesis, and pilot study had suggested potential microbiome differences between non-CA and CA individuals based on 16S rRNA gene sequencing. We here assess microbiome differences in a larger cohort of human subjects with and without CA, and among … Continue reading Permissive microbiome characterizes human subjects with a neurovascular disease cavernous angioma

Adverse effects of electronic #cigarettes on the disease-naive oral #microbiome

Six percent of Americans, including 3 million high schoolers, use e-cigarettes, which contain potentially toxic substances, volatile organic compounds, and metals. We present the first human study on the effects of e-cigarette exposure in the oral cavity. By interrogating both immunoinflammatory responses and microbial functional dynamics, we discovered pathogen overrepresentation, higher virulence signatures, and a brisk proinflammatory signal in clinically healthy e-cigarette users, equivalent to … Continue reading Adverse effects of electronic #cigarettes on the disease-naive oral #microbiome

Sputum #microbiome profiles identify severe #asthma phenotypes of relative stability at 12-18 months

..Data were available for 100 severe asthma subjects (median age: 55 yrs, 42% males). Two microbiome-driven clusters were identified, characterized by differences in asthma onset, smoking status, residential locations, percentage of blood and/or sputum neutrophils and macrophages, lung spirometry, and concurrent asthma medications (all p-values <.05). Cluster 2 patients displayed a commensal-deficient bacterial profile which was associated with worse asthma outcomes compared to cluster 1. … Continue reading Sputum #microbiome profiles identify severe #asthma phenotypes of relative stability at 12-18 months